Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics

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Authors
Rickman, Olivia J.
Voutsina, Nikol
Baple, Emma L.
Crosby, Andrew H.
Issue Date
2020-10-16
Type
Journal Article
Language
en
Keywords
Cholesterol , Liquid chromatography-mass spectrometry , Metabolism , Mitochondria , Oxysterol , Peripheral blood mononuclear cell , Wessex Classification Subject Headings::Biochemistry
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The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article "A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism" in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.
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Citation
Borah K et al. Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics. Data Brief. 2020 Oct 16;33:106382.
Publisher
Elsevier Science
License
© 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )
CC0 1.0 Universal
Journal
Data In Brief
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