Show simple item record

dc.contributor.authorCizmeci, D.en
dc.contributor.authorDempster, E. L.en
dc.contributor.authorChampion, O. L.en
dc.contributor.authorWagley, S.en
dc.contributor.authorAkman, O. E.en
dc.contributor.authorPrior, J. L.en
dc.contributor.authorSoyer, O. S.en
dc.contributor.authorMill, Jonathanen
dc.contributor.authorTitball, R. W.en
dc.date.accessioned2017-03-13T10:08:58Z
dc.date.available2017-03-13T10:08:58Z
dc.date.issued2016-08-03
dc.identifier.citationMapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection. 2016, 6:30861 Sci Repen
dc.identifier.issn2045-2322
dc.identifier.pmid27484700
dc.identifier.doi10.1038/srep30861
dc.identifier.urihttp://hdl.handle.net/11287/620271
dc.description.abstractThe potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. We assessed genome-wide patterns of DNA methylation in human macrophage-like U937 cells following infection with Burkholderia pseudomallei, an intracellular bacterial pathogen and the causative agent of human melioidosis. Our analyses revealed significant changes in host cell DNA methylation, at multiple CpG sites in the host cell genome, following infection. Infection induced differentially methylated probes (iDMPs) showing the greatest changes in DNA methylation were found to be in the vicinity of genes involved in inflammatory responses, intracellular signalling, apoptosis and pathogen-induced signalling. A comparison of our data with reported methylome changes in cells infected with M. tuberculosis revealed commonality of differentially methylated genes, including genes involved in T cell responses (BCL11B, FOXO1, KIF13B, PAWR, SOX4, SYK), actin cytoskeleton organisation (ACTR3, CDC42BPA, DTNBP1, FERMT2, PRKCZ, RAC1), and cytokine production (FOXP1, IRF8, MR1). Overall our findings show that pathogenic-specific and pathogen-common changes in the methylome occur following infection.en
dc.language.isoenen
dc.publisherNatureen
dc.relation.urlhttp://dx.doi.org/10.1038/srep30861en
dc.rightsArchived with thanks to Scientific reportsen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleMapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection.en
dc.typeJournal Articleen
dc.identifier.journalScientific reportsen
dc.description.noteThis article is freely available. Click on the Additional Link above to access the full-text.en
dc.type.versionPublisheden


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record