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    Directed evolution of aldolases for exploitation in synthetic organic chemistry.

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    URI
    http://hdl.handle.net/11287/619038
    Author
    Bolt, Amanda H.
    Berry, A.
    Nelson, A.
    Date
    2008-06-15
    Journal
    Archives of biochemistry and biophysics
    Type
    Research Support, Non-U.S. Gov't
    Publisher
    Elsevier
    DOI
    10.1016/j.abb.2008.01.005
    Rights
    Archived with thanks to Archives of biochemistry and biophysics
    Metadata
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    Abstract
    This review focuses on the directed evolution of aldolases with synthetically useful properties. Directed evolution has been used to address a number of limitations associated with the use of wild-type aldolases as catalysts in synthetic organic chemistry. The generation of aldolase enzymes with a modified or expanded substrate repertoire is described. Particular emphasis is placed on the directed evolution of aldolases with modified stereochemical properties: such enzymes can be useful catalysts in the stereoselective synthesis of biologically active small molecules. The review also describes some of the fundamental insights into mechanistic enzymology that directed evolution can provide.
    Citation
    Directed evolution of aldolases for exploitation in synthetic organic chemistry. 2008, 474 (2):318-30 Arch. Biochem. Biophys.
    Publisher URL
    http://www.sciencedirect.com/science/article/pii/S0003986108000167
    Note
    This article is freely available via Open Access. Click on the 'Additional Link' above to access the full-text from the publisher's site.
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